A Comparative Study of Ashokarishtam and Clomiphere Citrate in Combating Polycystic Ovary Syndrome Induced Oxidative Stress in Rat

نویسندگان

  • NAGARAJAN
  • NISHA
  • KISHOR RAJ
چکیده

Body has defense mechanisms against oxidants in the form of both enzymatic and non enzymatic antioxidants. Oxidants have both physiological and pathological role in female reproduction. Oxidative stress causes damage to the ovaries, oogonia and ovulation as well as in implantation procedures. A study was undertaken to evaluate the polycystic ovary syndrome (PCOS) induced stress in ovarian and uterine tissues and to compare the protective effect of clomiphene citrate and ashokarishtam. Elevated levels of malondialdehyde (37.99 ± 0.88 to 63.08 ± 1.09 mg/g/tissue) and myeloperoxidase (01.59 ± 0.14 to 02.61 ± 0.18 H202 lib/nm/mt/ mgptn) in ovary and uterus were observed in PCOS induced rat. After ashokarishtam treatment it displayed decreased levels of (p<0.001) malondialdehyde and myeloperoxidase in ovary (33.83 ± 0.69 mg/g/tissues) and uterus (01.12 ± 0.15 H2O2 lib/nm/mt/mgptn). However, clomiphene citrate treatment never resulted in such significant decrease of either malondialdehyde or myeloperoxidase. Study showed significant rise of reduced glutathione (p<0.001 level) in ovary and uterus after ashokarishtam treatment. Clomiphene citrate could increase the antioxidant only up to p<0.01 level. The study shows the extent of oxidative stress in ovarian and uterine tissues as indicated by increased levels of malondialdehyde and myeloperoxidase and decreased level of the antioxidant glutathione.

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تاریخ انتشار 2010